Study on saponins from Reineckia carnea belongs to Guizhou Miao medicine
10.7501/j.issn.0253-2670.2018.06.005
- Author:
Xu XU
1
Author Information
1. Jiangxi University of Traditional Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Aspidistrin A;
Ophiopogonin T;
Prosaikogenin D;
Reineckia carnea (Andr.) Kunth;
Saikosaponin b2;
Saponins
- From:
Chinese Traditional and Herbal Drugs
2018;49(6):1261-1265
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the saponins from Reineckia carnea of Guizhou Miao medicine. Methods: The chemical constituents from 95% EtOH extract were isolated and purified by repeated silica gel column chromatography, macroporous resin, ODS C18, Sephadex LH-20 gel column chromatography, and Pre-HPLC, and their structures were identified on the basis of physical and chemical properties, HR-ESI-MS and NMR spectral analysis. Results: Ten compounds were isolated and identified as (1β,3β,16β,22S)-cholest-5-ene-1,3,16,22-tetrol1,16-di (β-D-glucopyranosede) (1), (1β,3β,16β,22S)-cholest-5-ene-1,3,16,22-tetrol-1-[O-α-L-rhamno-pyranosyl-(1→2)-β-D-glucopyranoside]-16-(β-D-glucopyranoside) (2), (20S,22R)-spirost-25(27)-en-1β,3β,4β,5β-tetraol-5-O-β-D-glucopyranoside (3), kitigenin 5-O-β-D-glucopyrannoside (4), aspidistrin A (5), (17,20-S-trans)-5β-pregn-16-en-1β,3β-diol-20-one-1-O-β-D-xylopyranosyl-(1→2)-[α-L-rhamnopyranosyl]-3-O-α-L-rhamnopyranoside (6), diosgenin-3-O-β-D-glucopyranoside (7), ophiopogonin T (8), prosaikogenin D (9), and saikosaponin b2 (10). Conclusion: Compounds 3, 5, 8, 9, and 10 are isolated from this plant for the first time, which provided some reference for further elucidation and development of saponins from R. carnea.
