Application of QAMS method in quantitative evaluation of Xuefu Zhuyu Pills
10.7501/j.issn.0253-2670.2018.07.016
- Author:
Ling ZHANG
1
Author Information
1. Tianjin Huanhu Hospital
- Publication Type:Journal Article
- Keywords:
Paeoniflorin;
Quality evaluation;
Quantitative analysis of multi-components by single marker;
Relative correction factor;
Xuefu Zhuyu Pills
- From:
Chinese Traditional and Herbal Drugs
2018;49(7):1588-1593
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a quantitative analysis of multi-components by single marker (QAMS) method for the determination of ten components in Xuefu Zhuyu Pills (XZP), which are paeoniflorin, ferulic acid, amygdalin, liquiritin, catalpol, β-ecdysone, hydroxysafflor yellow A, naringin, neohesperidin, and saikosaponin A, and to examine the feasibilities of the method for quality control of XZP. Methods: Using XZP as the research object, paeoniflorin was chosen as the internal reference substance by correction methods. The relative correlation factors (f) of ferulic acid, amygdalin, liquiritin, catalpol, β-ecdysone, hydroxysafflor yellow A, naringin, neohesperidin, and saikosaponin A to paeoniflorin were established. The contents of ten components in 16 batches of samples were determined by the external standard method and QAMS, and the measured values of the external standard method were compared with the calculated results of correction factor in order to test the accuracy of established method. The results were compared with the methods finally. Results: The f value of ferulic acid, amygdalin, liquiritin, catalpol, β-ecdysone, hydroxysafflor yellow A, naringin, neohesperidin, and saikosaponin A to paeoniflorin were 2.22, 1.73, 0.56, 3.55, 2.03, 2.31, 1.03, 1.68, and 1.13, respectively; There was no significant difference between the calculated value and the measured value of f by correction method. The contents of nine batches of catalpol, amygdalin, saikosaponin A, ferulic acid, naringin, neohesperidin, liquiritin, β-ecdysone, hydroxysafflor yellow A, and paeoniflorin were 0.47-0.72, 0.63-0.94, 0.37-0.74, 0.11-0.33, 1.15-1.37, 0.64-0.84, 1.47-1.88, 0.38-0.62, 0.17-0.34, and 1.16-1.48 mg/g, respectively. Conclusion: The QAMS method is feasible and credible, and could be used to determine and control the multiple components in XZP.
