Study on chemical constituents and pharmacodynamic changes of Bufonis Venenum with different drying methods
10.7501/j.issn.0253-2670.2018.08.012
- Author:
Yinglan CHEN
1
Author Information
1. School of Pharmacy, Shanghai University of Traditional Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Arenobufagin;
Bufalin;
Bufonis Venenum;
Bufotalin;
Cinobufagin;
Cinobufotalin;
Drying method;
Process method;
Resibufogenin;
Telocinobufagin
- From:
Chinese Traditional and Herbal Drugs
2018;49(8):1816-1822
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the changes of chemical constituents and pharmacodynamics with different drying methods (sun- drying, 50 ℃ vacuum-drying, 50 ℃ and 80 ℃ heat-drying, freeze drying method) in Bufonis Venenum. Methods HPLC method and TLC method was established for studying the changes of chemical constituents from B. Venenum before and after being dried, and determine the inhibitory effect of dried samples on five different tumor cell lines proliferation by MTT assay. Results The B. Venenum processed by five different methods were different in character, while no significant differences in the types and content of chemical constituents; The total content of resibufogenin and cinobufagin was more than 6%, which was consistent with the 2015 edition of Chinese Pharmacopeia; 50 ℃ and 80 ℃ heat-drying of B. Venenum showed more effective inhibitiory effect than other dry methods. Conclusion The appearance of B. Venenum met the criterion of pharmacoperia by sun-drying and 50 ℃ heat-drying method. Although the color of B. Venenum under the vacuum-drying and freeze drying method did not meet the requirements of Chinese Pharmacopeia, the main effective components have a few changes.
