Assessment of genetic diversity and genetic structure of germplasm of wild Hedysari Radix based on ISSR markers
10.7501/j.issn.0253-2670.2018.10.027
- VernacularTitle: 基于ISSR的野生红芪种质遗传多样性与遗传结构研究
- Author:
Zheng-Ze QIANG
1
Author Information
1. Gansu University of Traditional Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Genetic diversity;
Genetic structure;
Germplasm of Hedysari Radix;
ISSR;
Wild
- From:
Chinese Traditional and Herbal Drugs
2018;49(10):2432-2439
- CountryChina
- Language:Chinese
-
Abstract:
Objective Assessment of genetic diversity and genetic structure of germplasm of wild Hedysari Radix. Methods Fifteen germplasms of wild Hedysari Radix. were collected from Wudu pupolations and Tanchang pupolations. ISSR (Intersimple sequence repeat) markers were used. ISSR data were analyzed with the program POPGEN 1.32. The UPGMA tree and PCoA analysis was constructed using Ntsys software. The AMOVA analysis used Arlequin 31 software and Population structure analysis used the structure 2.3.4 software. Results The results of the ISSR-PCR showed that 126 (72.83%) of the 173 ISSR locitested were polymorphic by the nine primers. The change of percentage of polymorphic loci (PPL) of wild germplasm populations were 49.71%-61.85%. The average of PPL was 55.78%. Two populations of species level of observed number of alleles (Na), effective number of alleles (Ne), Nei's gene diversity (H), and Shannon's information index was 1.728 3, 1.364 6, 0.224 2, and 0.334 5, respectively. Total gene diversity (Ht), gene diversity with provenances (Hs), the coefficient of gene differentiation (Gst), and estimate of gene flow from Gst (Nm) of cultivated and wild population were 0.223 0, 0.192 1, 0.138 9, and 3.099 4, respectively. The among-population component accounted for 16.36% of the total variation, while the within-population component accounted for 83.64%, and genetic variation occured mainly within the population. UPGMA analysis showed that 15 samples were clustered into two branches including Ⅰ and Ⅱ. PCoA and population structure analysis confirmed the partitioning results of the UPGMA clustering. Mantel correlation test showed that there existed middle-level correlations between the genetic differentiation and geographical distance. Conclusion Our studies showed that the genetic diversity of Hedysari Radix populations was at a higher level, the characteristics of genetic structure included genetic differentiation that occurs mainly within populations and perennials, which provides theoretical basis for protecting and utilizing germplasm of Hedysari Radix resources.
