Human Mesenchymal Stem Cell Transplantation Induces Sympathetic Nerve Sprouting and Reduces the Gap Junction With Potential Proarrhythmias in Dogs.
10.4070/kcj.2008.38.10.536
- Author:
Sook Kyoung KIM
1
;
Hui Nam PAK
;
Gwang Il KIM
;
Jae Hyung PARK
;
Yong Fu FANG
;
Hong Euy LIM
;
Byung Soo KIM
;
Chun HWANG
;
Young Hoon KIM
Author Information
1. Division of Cardiology, Department of Internal Medicine, Korea University College of Medicine, Seoul, Korea. hnpak@korea.ac.kr
- Publication Type:Original Article
- Keywords:
Mesenchymal stem cells;
Nerve growth factor;
Gap Junctions;
Ventricular tachycardia
- MeSH:
Animals;
Connexin 43;
Dogs;
Gap Junctions;
Heart;
Humans;
Incidence;
Mesenchymal Stem Cell Transplantation;
Mesenchymal Stromal Cells;
Nerve Growth Factor;
RNA;
RNA, Messenger;
Salicylamides;
Tachycardia, Ventricular;
Transplants;
Tyrosine 3-Monooxygenase;
Ventricular Fibrillation;
Ventricular Function
- From:Korean Circulation Journal
2008;38(10):536-543
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND AND OBJECTIVES: Although human mesenchymal stem cell (hMSC) transplantation has been known to improve ventricular function, the potential proarrhythmic effects have not yet been studied. MATERIALS AND METHODS: We monitored the heart rhythm of 6 dogs for 4 weeks after transplantation of hMSC (1x10(7), epicardial injection) (hMSC group) and in 5 Sham dogs after the injection of the vehicle alone. Cardiac sympathetic nerve sprouting {nerve growth factor (NGF)-beta; tyrosine hydroxylase (TH)} and gap junction expression {connexin (Cx) 43} were evaluated in 10 dogs (5 hMSC and 5 Sham) that survived longer than 4 weeks. RESULTS: The hMSC group expressed higher levels of NGF-beta messenger ribonucleic acid (mRNA) (56.0+/-66.8 fold; p<0.01) with TH+ sympathetic nerves (0.51+/-0.40 vs. 0.15+/-0.13% area; p<0.03) than the Sham control. In contrast, the hMSC group expressed lower levels of Cx43 mRNA (0.59+/-0.29 fold, p<0.001) and Cx43+ (1.64+/-1.79 vs. 2.12+/-1.07% area, p<0.001) than the Sham control. The incidences of ventricular fibrillation were 33.3% and 0% in the hMSC group and Sham control, respectively. One of the dogs with ventricular fibrillation (VF) in the hMSC group died suddenly. CONCLUSION: hMSC transplantation may be proarrhythmic since NGF-beta expression increased with cardiac sympathetic hyperinnervation and the expression of Cx43 and the gap junction decreased.
