Two new Diels-Alder type compounds from cell cultures of Morus alba
10.7501/j.issn.0253-2670.2018.10.015
- Author:
Wen-Yu DU
1
Author Information
1. College of Life Sciences, Beijing University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Cell suspension cultures;
Cytotoxicity;
Diels-Alder adduct;
Mongolicin H;
Morbilisin J;
Morus alba L.
- From:
Chinese Traditional and Herbal Drugs
2018;49(10):2345-2352
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the Diels-Alder adducts from cell suspension cultures of Morus alba. Methods A variety of column chromatography (CC) including silica gel CC, Sephadex LH-20 CC, C18 CC, and semi-preparative HPLC were used to separate Diels-Alder adducts from cell cultures of M. alba. Their structures were identified by physicochemical properties and various spectroscopic experiments, including MS, NMR, and ECD. Results Eight Diels-Alder adducts were obtained from the ethyl acetate extract of M. alba, and determined as mongolicin H (1), morbilisin J (2), mongolicin F (3), mulberrofuran G (4), artonin D (5), kuwanon R (6), morbilisin C (7), and mulberrofuran E (8). Conclusion Compounds 1-8 are all Diels-Alder adducts and show medium cytotoxic activity, and compounds 1 and 2 are new compounds.
