Applications progress of stable isotopic tracer technique in metabolic regulation of endogenous substances
10.7501/j.issn.0253-2670.2018.11.029
- Author:
Ting LINGHU
1
Author Information
1. Modern Research Center for Traditional Chinese Medicine, Shanxi University
- Publication Type:Journal Article
- Keywords:
Endogenous substances;
Metabolic flux analysis;
Metabolic pathway;
Metabolic regulation;
Stable isotope
- From:
Chinese Traditional and Herbal Drugs
2018;49(11):2678-2685
- CountryChina
- Language:Chinese
-
Abstract:
With the rapid development of high-sensitivity detection methods, stable isotope tracing technique has received increasing attention. Stable isotope tracing technique can accurately track the activity of labeled compounds in the body through the tracer atoms and determine their specific metabolic pathways based on the distribution of isotopic peaks of the intermediate metabolites. By calculating the flux, the metabolic pathways are analyzed to provide a basis for the study of disease mechanism and drug metabolism. In recent years, the technique has a wide application in the field of biomedicine. This paper summarizes the applications of stable isotopic tracer technique in the metabolic regulation of endogenous substances such as carbohydrate metabolism, lipid metabolism, amino acid metabolism, hormone metabolism, nucleic acid metabolism, and so on.
