Mechanism analysis of Salviae Miltiorrhiza and Ligustrazine Hydrochloride Injection based on network pharmacology
10.7501/j.issn.0253-2670.2018.11.018
- Author:
Qian YANG
1
Author Information
1. Ri Zhao Hospital of TCM
- Publication Type:Journal Article
- Keywords:
Enrichment analysis;
Network pharmacology;
Protein-protein interaction;
Salviae Miltiorrhiza and Ligustrazine Hydrochloride Injection;
Targets
- From:
Chinese Traditional and Herbal Drugs
2018;49(11):2606-2613
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the efficacy and mechanism of Salviae Miltiorrhiza and Ligustrazine Hydrochloride Injection (SMLHI). Methods The targets of chemical components of SMLHI were predicted and the compounds-targets (C-T) network was constructed. The key targets were screened through the topology analysis of the C-T network. Also, protein-protein interaction (PPI) network was established, and the Gene ontology and KEGG pathway was enriched and analyzed. The pharmacological action mechanism of SMLHI was predicted, and the mechanism was preliminarily verified by pretreating with SMLHI on the MARK expression of cerebral ischemia rats. Results Fifteen main compounds in SMLHI act on 94 targets and PRSS1, PTGS2, F2, and PTGS1 were key targets in the C-T network. There were 71 targets in the PPI network including several key nodes such as SRC, MAPK-1, MMP-9, MAPK-14, PTGS2, BCL-2, and so on. All the targets were enriched in 26 GO items and six KEGG pathways. Conclusion Results in this study preliminarily verified the action of SMLHI on cerebral infarction and diabetic peripheral neuropathy, thus laying a solid foundation for further study on the mechanism of action.
