Optimization of prescription and preparation technology of brucine nanostructured lipid carriers
10.7501/j.issn.0253-2670.2018.11.011
- Author:
Qing-Xia GUAN
1
Author Information
1. College of Pharmacy, Heilongjiang University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Brucine;
Central composite design-response surface method;
Nanostructured lipid carriers;
Single factor method;
Solvent emulsification ultrasonic method
- From:
Chinese Traditional and Herbal Drugs
2018;49(11):2557-2563
- CountryChina
- Language:Chinese
-
Abstract:
Objective To optimize the prescription and preparation technology of brucine nanostructured lipid carriers (B-NLC). Methods The method of "the solvent emulsification ultrasound" was used to prepare B-NLC. The prescription and preparation was optimized using a single factor method combined with central composite design-response surface methodology (CCD-RSM). Results The resultant B-NLC was transparent liquid with light blue opalescence. The optimal conditions were that the dosage of drugs was 1.28 mg, the mass concentration of poloxamer 188 was 1.08%, and the ratio of solid lipid to liquid lipid was 1.45:1. The obtained NLC showed the average particle size of (136.89 ± 4.23) nm with a polydispersity index of 0.289 ± 0.005 and a zeta potential of (-34.46 ± 0.31) mV. The entrapment efficiency was calculated to be (68.98 ± 2.06)%, and the drug loading content was (1.90 ± 0.06)%. Conclusion B-NLC prepared by solvent emulsification ultrasound had a high entrapment efficiency and a narrow particle size distribution. The method was easy and simple and can be used to optimize the prescription and preparation of B-NLC, which provides a foundation for the further in vivo research of brucine.
