Preparation process of galactosylated liposome of modified cantharidin
10.7501/j.issn.0253-2670.2018.12.012
- Author:
Man-Shu ZOU
1
Author Information
1. College of Pharmacy, Hunan University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Cantharidin;
Encapsulation rate;
Film dispersion method;
Galactosylated liposomes;
GC-MS;
Orthogonal test;
Preparation process
- From:
Chinese Traditional and Herbal Drugs
2018;49(12):2809-2816
- CountryChina
- Language:Chinese
-
Abstract:
Objective To optimize the formulation ratio and preparation process of galactosylated cantharidin liposome (Lac-CTD- lips) and establish its methodology for content determination. Methods The method of determination of GC-MS cantharidin content was established by film dispersion method. The entrapment efficiency of cantharidin was evaluated as an index. The preparation process of Lac-CTD-lips was optimized by single factor and orthogonal experiments. Its surface characteristics, encapsulation efficiency, particle size, and Zeta potential were also investigated. Results The best prescription was as follow: cantharidin: hydrogenated soya lecithin:cholesterol at 1:20:5, 10% galactoside, film-forming at 50 ℃, film cleaning with 30 mL of PBS solution of pH 6.0, and hydartion at 40 ℃ for 1.5 h. The resulting liposomes exhibited a pale blue opalescent appearance, a spherical particle morphology, and a more rounded surface with no adhesion. The average particle size was (123.9 ± 4.8) nm (n = 3), the particle size distribution was single-peak, the zeta potential was (-0.36 ± 0.81) mV (n = 3), and the encapsulation efficiency was over 75%. Conclusion GC-MS is suitable for the determination and analysis of cantharidin content. The optimal preparation technology from orthogonal experiment is stable and reliable. The obtained liposomes have higher encapsulation efficiency, small particle size, and good appearance.
