Callus induction and establishment of plant regeneration system of Saposhnikovia divaricate
10.7501/j.issn.0253-2670.2018.13.026
- Author:
Hang FU
1
Author Information
1. College of Chinese Medicine Materials, Jilin Agricultural University
- Publication Type:Journal Article
- Keywords:
Callus;
Plant regeneration;
Response surface test design;
Saposhnikovia divaricate (Turcz.) Schisck.;
Tissue cultures
- From:
Chinese Traditional and Herbal Drugs
2018;49(13):3127-3133
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish the regeneration system of Saposhnikovia divaricate (Turcz.) Schisck. Methods: The effects of different explants, temperature, medium, and illumination was studied on the induction of S. divaricate. Response surface test design was used to optimize the hormone ratio of callus induction and proliferation. Results: The leaves of S. divaricate were used as the experimental materials, the optimal medium for the callus induction was MS + 6-BA 0.90 mg/L + 2,4-D 1.05 mg/L + KT 0.96 mg/L, with temperature of 25 ℃ and light of 12 h/d. The optimal medium for callus proliferation was MS + 6-BA 0.56 mg/L + 2,4-D 0.48 mg/L + KT 0.40 mg/L, and the subculture cycle was 35 d. The optimal medium for adventitious shoots was MS + NAA 0.6 mg/L + 6-BA 1.0 mg/L. The optimal medium for root growing was MS + NAA 0.4 mg/L + 6-BA 0.1 mg/L. Conclusion: S. divaricate callus was successfully induced and a stable and efficient plant regeneration system was established.
