ISSR analysis of genetic diversity and relationship among medicinal plants of Epimedium acuminatum from different habitats in Guizhou Province
10.7501/j.issn.0253-2670.2018.13.025
- Author:
Jian-Ming SHI
1
Author Information
1. College of Life Sciences, Guizhou University
- Publication Type:Journal Article
- Keywords:
Epimedium acuminatum Franch.;
Genetic diversity;
Genetic relationship;
ISSR;
UPGMA method
- From:
Chinese Traditional and Herbal Drugs
2018;49(13):3122-3126
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To analyze the genetic diversity and relationship of Epimedium acuminatum from different habitats in Guizhou Province. Methods: PopGene 32 software and NTsys2.10e software was used to analyze the genetic diversity and phylogenetic relationship of five different habitats in Guizhou Province, and phylogenetic tree was constructed according to the UPGMA method. Results: A total of 13 polymorphic and clear bands were screened from 100 random primers showed that amplified 211 bands, of which 205 were polymorphic from the level of species, the percentage of polymorphic bands (PPB) was 97.16%. The Shannon’s information index (I) was 0.455 7; Nei’s gene diversity index (He) was 0.297 3; The effective number (Ne) of alleles was 1.490 2; The total genetic diversity (Ht) of five populations was 0.297 3, while the population genetic diversity within the group (Hs) was 0.207 5, indicated that the genetic variation mainly existed within populations, genetic differentiation within population than that of population between. The population genetic distance clustering was built by UPGMA. The results showed that five populations were divided into two branches, three populations from Anshun, Huaxi, and Gaopo clustered into one branch, and the other two populations from Longli and Leishan populations clustered into one branch. Conclusion: ISSR molecular marker technique can be used to analyze the genetic diversity and phylogenetic relationship of Epimedium acuminatum from different habitats in Guizhou Province.
