Identification of chemical compositions in Chenxiang Huaqi Pills by UPLC-Q-TOF/MS
10.7501/j.issn.0253-2670.2018.13.006
- Author:
Xuan-Xuan PAN
1
Author Information
1. College of Pharmacy, Guangdong Pharmaceutical University
- Publication Type:Journal Article
- Keywords:
2-(2-phenylethyl) chromones;
Chenxiang Huaqi Pills;
Flavonoids;
Triterpenoid saponins;
UPLC-Q-TOF/MS
- From:
Chinese Traditional and Herbal Drugs
2018;49(13):2985-2992
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To analyze the main chemical constituents of traditional Chinese medicine compound Chenxiang Huaqi Pills by using UPLC-Q-TOF/MS technology. Methods: The separation was performed on Phenomenex Kinetex C18 column (100 mm×4.6 mm, 2.7 μm), and the gradient elution of acetonitrile-0.1% formic acid was used as mobile phase at a flow rate of 0.8 mL/min. The data was collected by the positive and negative ion modes using Q-TOF/MS and ESI source. The main chemical constituents of Chenxiang Huaqi Pills were identified according to the exact molecular mass, the cleavage fragments of MS/MS, the literature data, and the reference control. Results: A total of 73 chemical components were separated and identified in Chenxiang Huaqi Pills, including 36 flavonoids, 16 2-(2-phenylethyl) chromones, 7 triterpenoid saponins, 2 sesquiterpene lactones, and 12 other components. Conclusion: This study showed that UPLC-Q-TOF/MS technology provided a simple, rapid, and accurate method for the identification of chemical constituents in Chenxiang Huaqi Pills, which provided a new technology method for the pharmacological basis and quality control of Chenxiang Huaqi Pills.
