Genetic diversity of cultivated Gardenia jasminoides germplasms detected by SCoT markers
10.7501/j.issn.0253-2670.2018.14.025
- VernacularTitle: 基于SCoT标记的栽培栀子种质资源遗传多样性研究
- Author:
Yuan PAN
1
Author Information
1. Chongqing Academy of Chinese Material Medica
- Publication Type:Journal Article
- Keywords:
Gardenia jasminoides Ellis;
Genetic diversity;
PCR;
SCoT;
UPGMA method
- From:
Chinese Traditional and Herbal Drugs
2018;49(14):3376-3381
- CountryChina
- Language:Chinese
-
Abstract:
Objective: The genetic variation and relationship of 47 pieces of Gardenia jasminoides germplasms were analyzed by SCoT molecular markers. Methods: The genetic similarity coefficient was calculated by NTSYS version 2.1 software and the dendrogram was constructed by UPGMA method. Results: More than 20 polymorphic primers were screened from 36 primers and PCR amplificated to test materials. The primers generated totally 154 bands among which 120 bands were found to be polymorphic, with an average of 78.14%. The genetic similarity coefficient among the germplasms ranged from 0.655 8 to 0.980 5, and the average content was 0.784 1. The clustering results showed that the genetic diversity of G. jasminoides was rich and the genetic relationship was complex. Conclusion: SCoT markers were feasible and effective to analyze the genetic diversity of G. jasminoides germplasm resources. The results provide a reliable theoretical basis for breeding, classification, preservation and utilization of G. jasminoides.
