Cloning and prokaryotic expression of CDS region from GGPPS gene of Panax notoginseng
10.7501/j.issn.0253-2670.2018.15.028
- Author:
Mei-Qiong TANG
1
Author Information
1. Guangxi Key Laboratory of Medicinal Resources Conservation and Genetic Improvement, Guangxi Botanical Garden of Medicinal Plants
- Publication Type:Journal Article
- Keywords:
Clone;
Coding sequence;
Geranylgeranyl pyrophosphate synthase (GGPPS) gene;
Panax notoginseng (Burk.) F. H. Chen;
Prokaryotic expression
- From:
Chinese Traditional and Herbal Drugs
2018;49(15):3667-3671
- CountryChina
- Language:Chinese
-
Abstract:
Objective: In order to study the function of geranylgeranyl pyrophosphate synthase (GGPPS) gene, the CDS nucleotide sequence of GGPS was cloned from Panax notoginseng, and its prokaryotic expression was performed. Methods: The primers were designed according to the reported GGPPS gene sequence in Genbank, and the coding sequence was obtained by RT-PCR. The prokaryotic expression vector was constructed and transformed into Escherichia coli BL21 for the expression under the induction of isopropyl β-D-1-thiogalactopyranoside (IPTG). Results: The CDS of GGPS gene had a full length of 1 032 bp coding for 343 amino acids. Results of SDS-PAGE showed that a 29 000—44 000 protein was achieved and the recombinant protein was mainly in the form of insoluble inclusion body. Conclusion: The CDS nucleotide sequence of GGPPS gene was successfully cloned, and the stable prokaryotic expression was established. This study will provide a foundation for the further functional researches of GGPPS gene in P. notoginseng.
