Preparation of plumbagin transfersomal gel and investigation on its transdermal penetration characteristics in vitro
10.7501/j.issn.0253-2670.2018.15.022
- Author:
Shan-Shan MA
1
Author Information
1. School of Pharmacy, Southwest Minzu University
- Publication Type:Journal Article
- Keywords:
Central composite design and response surface method;
Film-ultrasonic dispersion method;
Gel;
Orthogonal test;
Plumbagin;
Transdermal delivery;
Transfersome
- From:
Chinese Traditional and Herbal Drugs
2018;49(15):3625-3631
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To prepare plumbagin transfersomal (PBG-T) gel and investigate its transdermal penetration characteristics in vitro. Methods: Plumbagin transfersomes were prepared by film-ultrasonic dispersion method. The optimal prescription condition of PBG-T was selected by central composite design and response surface method. The formula of PBG-T gel was optimized by orthogonal test. The Franz diffusion cell was used to investigate transdermal penetration characteristics of PBG-T gel in vitro. Results: The optimal prescription condition of transfersomes was determined as drug 10.0 mg, phospholipids 700.0 mg, Tween-80 91.5 mg, ultrasonication time 13 min. The optimal prescription condition of transfersomal gel was 1% carbomer 940 as gel matrix, and 5% glycerol as the humectant. According to the optimized prescription, the entrapment efficiency, the mean particle size, and Zeta potential of PBG-T were (79.88 ± 2.26)%, (125.64 ± 4.54) nm, and (-30.97 ± 1.13) mV. The cumulative penetration rate of PBG-T gel was 70.0% at 12 h. Conclusion: The optimal preparation technique is stable and feasible. Transfersomal gel features a sustained release in vitro, the transfersomal gel can increase penetration rate of plumbagin through the skin of rats.
