UPLC-Q/TOF method for simultaneous qualitative and quantitative analysis of main chemical constituents in medicinal materials of Sinodielsia yunnanensis
10.7501/j.issn.0253-2670.2018.15.016
- Author:
Wei-Han QIN
1
Author Information
1. Chongqing Academy of Chinese Materia Medica
- Publication Type:Journal Article
- Keywords:
1-O-β-D-glucopyranosyl-1,3-octanediol;
2-pentylbutanedioic;
6-(2-methoxyvinyl)-7-methyl-2H-1-benzopyran-2-one;
Ferulic acid;
Simultaneous qualitative and quantitative analysis;
Sinodielsia yunnanensis Wolff;
UPLC-Q/TOF
- From:
Chinese Traditional and Herbal Drugs
2018;49(15):3576-3582
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish UPLC-Q/TOF method for simultaneous qualitative and quantitative analysis of main chemical components in Sinodielsia yunnanensis. Methods: High performance liquid chromatography tandem flight time mass spectrometry was used. Chromatographic column was Agilent shell 120 Hilic, and the flow phase was acetonitrile-0.1% acid aqueous solution (gradient elution) at a flow rate of 0.3 mL/min; Qualitative and quantitative analysis adopted Q-TOF positive anion full scanning + IDA (information correlation acquisition) mode. Results: Using the established screening database, the target compounds with a mass error of less than 5×10-6, a correct isotope distribution and fragment ion are used as the target compounds. Combined with software Formula Finder, Mass Calculators and other functions, online database (Human Metabolome Database, PubChem, Chemical Book, etc.), and two stage fragmentation rule, 23 compounds were identified by qualitative identification. Ferulic acid was determined as a quantitative indicator component, and the quantitative and qualitative ions were 178 and 149, respectively; The linear range was 1.14-1 140 ng/mL (r = 1.000), and the limits of detection and quantitation respectively was 0.87 ng/mL and 2.91 ng/mL; RSDs of precision, stability, and reproducibility tests were lower than 2%; The recovery rate was 98.13%-101.25% (RSD = 1.37%, n = 5). Conclusion: This method has high sensitivity, good reproducibility, and its analysis is fast, accurate, and reliable, and it can be used for simultaneous qualitative and quantitative determination of main chemical components in the medicinal materials of S. yunnanensis; The content of ferulic acid in Yunnan is higher than that in Tibet.
