Inhibition of Wulong Xiaozheng Pill on migration and invasion of gastric cancer BGC-823 cells and its mechanism
10.7501/j.issn.0253-2670.2018.16.010
- Author:
Xue-Ying GUO
1
Author Information
1. Heilongjiang Academy of Traditional Chinese Medicine
- Publication Type:Journal Article
- Keywords:
BGC-823 cells;
Invasion;
Migration;
PI3K/Akt/NF-κB signaling pathway;
Wulong Xiaozheng Pill
- From:
Chinese Traditional and Herbal Drugs
2018;49(16):3788-3796
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Wulong Xiaozheng Pill (WXP) on the migration and invasion of human gastric cancer cell-line BGC-823 and its mechanism. Methods WXP, IGF-1, and LY294002 were added on BGC-823 cells. Then the inhibitory effect of WXP was detected by MTT assay. Transwell assay was performed to determine the migration and invasion capacity of on BGC-823 cells. Expressions of VEGF, MMP-2, and MMP-9 were detected by ELISA, while the expressions of related proteins and mRNA in PI3K/NF-κB signaling pathway were detected by Western blotting and RT-PCR. Results WXP can inhibit the proliferation, adhesion, invasion, and migration of BGC-823 cells. In addition, WXP inhibited the expression of VEGF, MMP-2 and MMP-9 protein in BGC-823 cells. WXP significantly inhibited the expression of p-PI3K, p-Akt, p-IKK-a, and p-NF-κB p65Ser 276 proteins, PI3K, Akt, IKKa, and NF-κB mRNA, which showed a time-dependent and dose-dependent manner. Conclusion WXP inhibit the capacity, migration and invasion of BGC-823 cells by blocking PI3K/NF-κB signaling pathway.
