Effect of ginsenoside Rh2 on drug resistance sensitivity of human gastric cancer SGC7901/ADR cells
10.7501/j.issn.0253-2670.2018.17.022
- Author:
Shuang-Shuang HU
1
Author Information
1. Shaanxi University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Adriamycin;
Chemosensitivity;
Drug resistance;
Ginsenoside Rh2;
SGC7901/ADR cells
- From:
Chinese Traditional and Herbal Drugs
2018;49(17):4113-4117
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of ginsenoside Rh2 (G-Rh2) on the proliferation, cell cycle and chemotherapy sensitivity of human gastric cancer cell line SGC7901/ADR. Methods MTT assay was used to detected the effects of G-Rh2 and adriamycin (ADR) on the proliferation of drug-resistant SGC7901/ADR cells and to calculate the reversal fold (RF). Flow cytometry was selected to detect The effects of G-Rh2 on cell cycle. the effects of G-Rh2 on the expression of P-gp and Bcl-2 proteins in drug-resistant SGC7901/ADR cells was detected by Western blotting. Results Compared with the IC50 value (54.52 μmol/L) after the ADR single drug treatment, the IC50 value (30.14 μmol/L) of the cells treated with G-Rh2 and ADR decreased significantly, and RF was 1.81. G-Rh2 combined with ADR arrested the cell cycle in G2/M phase and significantly decreased the protein expression of P-gp and Bcl-2(P < 0.05). Conclusion G-Rh2 combined with ADR could increase the chemosensitivity of SGC7901/ADR cells, which may inhibit the proliferation of gastric cancer cells by blocking cell cycle and increasing apoptosis.
