Comparation of chemical composition of Cyathula offinalis between different growth years by HPLC characteristic fingerprint combined with chemometrics
10.7501/j.issn.0253-2670.2018.22.027
- Author:
Chong-Jing SHI
1
Author Information
1. Chengdu University of Traditional Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Characteristic fingerprint;
Chemical composition differences;
Chemometrics;
Cyathula offinalis Kuan;
Growth years;
HPLC
- From:
Chinese Traditional and Herbal Drugs
2018;49(22):5404-5409
- CountryChina
- Language:Chinese
-
Abstract:
Objective HPLC characteristic fingerprint and chemometrics method were used to compare the chemical characteristics difference between 1-6 years old Cyathula offinalis. Methods The peak area data matrix of 6 × 54 and the difference of characteristic peak of 1-6 years old C. offinalis were analyzed by HPLC. Similarity analysis the samples was investigated with “Similarity Evaluation System for Chromatographic Fingerprint of Chinese Materia Medica 2004A”. Hierarchical clustering analysis (HCA) and principal component analysis (PCA) were carried out with SPSS 22.0 software. Results According to the analysis of characteristic peak difference, 1 and 6 years old C. offinalis showed exclusive difference in four chromatographic peaks and five chromatographic peaks, respectively. The peak area of 1 year old sample was significantly higher than other years in four chromatographic peaks, which showed obvious differences in components cumulant. The similarity degree analysis showed that the similarity degree of the 3, 4, and 5 years old C. offinalis were more than 0.98, but the 1, 2 and 6 years old were less than 0.85. Dendrogram of HCA and 3D scatter plot of PCA showed that the 3, 4, and 5 years old C. offinalis were clustered into one class. Conclusion There are certain differences in the chemical components among different growth years of C. offinalis. The chemical components of the 3, 4, and 5 years old C. offinalis tend to be uniform, which provides a scientific reference for the harvest time of C. offinalis.
