Study on chemical constituents from Xiaoer Fupi Granules based on UPLC-LTQ-Orbitrap-MS
10.7501/j.issn.0253-2670.2018.23.007
- Author:
Hong-Ping LONG
1
Author Information
1. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences
- Publication Type:Journal Article
- Keywords:
Component analysis;
Pharmacodyamic material basis;
Polymethoxyflavones;
UPLC-LTQ-Orbitrap-MS;
Xiaoer Fupi Granules
- From:
Chinese Traditional and Herbal Drugs
2018;49(23):5522-5531
- CountryChina
- Language:Chinese
-
Abstract:
Objective To clarify the chemical constituents from Xiaoer Fupi Granules and establish an effective identification method for the complex composition of Chinese material medica. Methods UPLC-LTQ-Orbitrap-MS was used to analyze the chemical constituents of Xiaoer Fupi Granules. The analysis was performed on Waters HSS T3-C18 (100 mm × 2.1 mm, 1.8 μm). The mobile phase consisted of 0.1% ammonium formate (A)-acetonitrile (B), which was used for gradient elution, with the flow rate of 0.3 mL/min. This objective was achieved mainly depending on the information of the accurate mass and the multistage fragment ions obtained by UPLC-LTQ-Orbitrap-MS, comparing the mass spectrometric data of the standard substance and consulting the reference literatures. Results A total of 84 compounds were identified in this study, including the flavones, glycosidics, phenoliacids, and its esters, and so on, which were attributed the herbs source of each compound. Meanwhile, an effective and quickly identification method for the glycosides, flavonoids, and polyoxyflavonoids were established based on the law of multistage mass spectrometry. Conclusion The comprehensive chemical constituents analysis of Xiaoer Fupi Granules will provide the scientific theory basis for the pharmacodyamic material basis and the quality control of this drug.
