Exploration and application of a new method for rapid extraction of DNA from Chinese medicinal materials
10.7501/j.issn.0253-2670.2019.02.032
- Author:
Lu YANG
1
Author Information
1. School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Chinese medicinal materials;
DNA extraction;
Filter paper;
Nucleic acid;
Rapid extraction
- From:
Chinese Traditional and Herbal Drugs
2019;50(2):502-509
- CountryChina
- Language:Chinese
-
Abstract:
Objective To build an equipment-free nucleic acid extraction dipstick methodology that can obtain amplification-ready DNA from Chinese medicinal materials in 30 s. Methods A new rapid DNA extraction method which was suitable for traditional Chinese medicine, with equipment-free nucleic acid extraction dipstick methodology that can obtain amplification-ready DNA from Chinese medicinal materials in 30 s was verified. The filter paper strip or disk for the adsorbed nucleic acid can be directly amplified by the reaction system. Results This method can extract the nucleic acid successfully. The extracted DNA was amplified, and the results were consistent with the traditional extraction methods. Conclusion The new rapid DNA extraction method is simple, fast, and low cost, which will make nucleic acid extraction become more and more popular, and no longer confined to professional and laboratory environment. This study provides a new idea for the application and development of molecular pharmacology.
