Molecular mechanism of Erchen Decoction in treatment of coronary heart disease based on network pharmacology
10.7501/j.issn.0253-2670.2019.02.025
- Author:
Wen-Na CHEN
1
Author Information
1. Center of Teaching and Laboratory, Liaoning University of Traditional Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Coronary heart disease;
Erchen Decoction;
Metabolites;
Network pharmacology;
Targets prediction
- From:
Chinese Traditional and Herbal Drugs
2019;50(2):441-448
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the targets, metabolites, and signal pathways of Erchen Decoction based on network pharmacology and to reveal the molecular mechanism of Erchen Decoction in the treatment of coronary heart disease (CHD) by “phlegm and blood stasis”. Methods TCMSP, TCM-PTD, and TCMID were used to find the main ingredients of Erchen Decoction. Targets of Erchen Decoction were predicted by using ChemMapper database. The targets related with coronary heart disease were obtained by searching through TTD, Drugbank, and DisGeNET databases. The common targets of disease-drug component were selected by intersecting disease targets with drug targets. A protein-protein interaction (PPI) network model was constructed using the STRING platform. The metabolites of above-mentioned and selected components were analyzed using the MetaboAnalyst database to screen out the main metabolites. MetScape and ClueGO APP in Cytoscape were used to analyze the gene function and metabolic pathway of Erchen Decoction in the treatment of CHD, and to construct the main drug component-metabolite-target-signal pathway topology network of Erchen Decoction. Results Eight main drug ingredients of Erchen Decoction, 56 predicted targets related with CHD, and 13 metabolites and 13 signal pathways related to the main components of Erchen Decoction were screened out by network analysis at the same time. The molecular network of compounds of Erchen Decoction-metabolites-CHD targets-signal pathways were constructed based on all the targets. The results showed that Erchen Decoction may play a therapeutic role in CHD by protecting vascular endothelium, anti-oxidative stress, anti-inflammatory and injury repair by multi-targets and multi-pathways. Conclusion This research explores the possible molecular mechanism of Erchen Decoction in the treatment of CHD by “phlegm and blood stasis” and reveals the pharmacological effects of Erchen Decoction to provide direction for carrying out the follow-up evaluation of curative effect of Erchen Decoction in clinic.
