Effects of promoting osteogenic differentiation of rBMSCs and ROBs using five kinds of flavonoids from Hedysari Radix
10.7501/j.issn.0253-2670.2019.03.015
- Author:
Yao-Yao FANG
1
Author Information
1. College of Pharmacy, Lanzhou University
- Publication Type:Journal Article
- Keywords:
Bone;
Calycosin;
Formononetin;
Hedysari Radix;
Isoliquiritigenin;
Medicarpin;
mesenchymal stem cells;
Ononin;
Osteoblasts;
Osteogenic differentiation
- From:
Chinese Traditional and Herbal Drugs
2019;50(3):632-638
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effects of five kinds of flavonoids (calycosin, formononetin, ononin, isoliquiritigenin, and medicarpin) from Hedysari Radix on promoting osteogenic differentiation of rat bone marrow stromal cells (rBMSCs) and rat calvarial osteoblasts (ROBs). Methods: rBMSCs were isolated according to plastic adherence. ROBs were isolated by enzyme digestion method. The proliferation of rBMSCs and ROBs were detected by MTT assay. ALP activity and calcium content of rBMSCs and ROBs cells were detected by alkaline phosphatase kit and calcium kit. Mineralized nodule formation was detected by alizarin red staining. Results: The five components could promote proliferation, increase ALP activity, increase calcium content, and increase the area and number of calcified nodules of rBMSCs and ROBs (P < 0.05). Among them, calycosin had the best effect on promoting the osteogenic differentiation of rBMSCs, and medicarpin promoted the osteogenic differentiation of ROBs with the best effect, followed by calycosin. Conclusion: Five flavonoids promoted the improvement of osteogenic function, while calycosin has better osteogenic activity on rBMSCs and ROBs and can be used as an excellent osteoinductive factor.
