Anti-oxidant components in Scutellaria baicalensis based on HPLC-ABTS-DAD-Q-TOF/MS
10.7501/j.issn.0253-2670.2019.03.006
- Author:
Yu-Qing WANG
1
Author Information
1. Research Center, Nanyang Medical College
- Publication Type:Journal Article
- Keywords:
5,7,2’-trihydroxy-6-methoxyflavone anti-oxidant effect;
Baicalin;
Flavonoids;
HPLC-ABTS-DAD-Q-TOF/MS;
Scutellaria baicalensis Georgi
- From:
Chinese Traditional and Herbal Drugs
2019;50(3):568-572
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a two-dimensional fingerprint of Scutellaria baicalensis between characteristic chemical composition and anti-oxidant activity, and to study its anti-oxidant constituents on-line. Methods: The scavenging activities of S. baicalensis for ABTS+ by HPLC-ABTS-DAD-Q-TOF/MS was studied and its active constituents were screened. A total of ten anti-oxidant components in S. baicalensis were identified. The anti-oxidant activities of different active ingredients were evaluated by anti-oxidant efficiency. Results: The data showed that baicalin, 5,7,2’,5’-tetrahydroxy-8,6’-dimethoxy flavone, 5,7,2’- trihydroxy-6-methoxyflavone, and baicalein had stronger anti-oxidant activity, and 6-C-arabinosyl-8-C-glucosylchrysin, 6-C-glucosyl-8-C-arabinosyl-chrysin, oroxyloside, chrysin-7-O-glucuronide, wogonoside, and norwogonin had less anti-oxidant activity. Conclusion: HPLC-ABTS-DAD-Q-TOF/MS method can rapidly analyze and identify the anti-oxidant components in S. baicalensis, and these experimental data can provide support for the basic research of the anti-oxidant ingredients of S. baicalensis.
