Metabolites from Cercospora lagenariae MT-45, an endophytic fungus isolated from Huperzia serrata
10.7501/j.issn.0253-2670.2019.03.002
- Author:
Yun WU
1
Author Information
1. Modern Research Center for Traditional Chinese Medicine, School of Chinese Materia Medica, Beijing University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Alternariol;
Cercolagenlic acid A;
Cercospora;
Endophytic fungi;
Huperzia serrata (Thunb.) Trev;
Inhibitory activity against nitric oxide production;
Polyketide
- From:
Chinese Traditional and Herbal Drugs
2019;50(3):546-550
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the chemical metabolites from Cercospora lagenariae MT-45, an endophytic fungus isolated from Huperzia serrata (Thunb.) Trev. Methods: The compounds were isolated and purified by using silica gel column chromatography and semi-preparative liquid chromatography. The structures were established using physicochemical properties and MS and NMR. The anti-inflammatory activities of all the isolates were also preliminarily investigated by using in vitro model. Results: Nine polyketide derivatives including cercolagenlic acid A (1), alternariol (2), alternariol 9-methyl ether (3), (+)-nigrosporaol A (4), alternarienonic acid B (5), 2-methyl-5-carboxymethyl-7-hydroxychromone (6), 2,5-dimethyl-7-hydroxychromone (7), 1-deoxy- rubralactone (8), and (-)-alternarlactam (9) were isolated from C. lagenariae MT-45 fermented on brown rice solids. Conclusion: Compound 1 is a new compound, and compound 6 can exhibit a certain inhibition on the nitric oxide production in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells with an IC50 value of (57.5 ± 1.2) μmol/L.
