Cloning of ERF1 transcription factor from Carthamus tinctorius and construction of plant expression vector
10.7501/j.issn.0253-2670.2019.04.026
- Author:
Xiu-Ming LIU
1
Author Information
1. Engineering Research Center of Bioreactor and Pharmaceutical Development of Ministry of Education, Jilin Agricultural University
- Publication Type:Journal Article
- Keywords:
AP2/ERF transcription factor;
Carthamus tinctorius L.;
Flavoniods synthesis;
Gene cloning;
Plant expression vector
- From:
Chinese Traditional and Herbal Drugs
2019;50(4):963-969
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To clone a coding region sequence of AP2/ERF transcription factor family from Carthamus tinctorius, and construct a plant expression vector. Methods: A gene (CtERF1) of AP2/ERF family transcription factor was cloned by RT-PCR based on the sequence of C. tinctorius transcription sequencing, the phylogenetic tree was constructed by ClustalW 1.83 software, Spe I and Xba I restriction sites were introduced to construct over-expression vector pBASTA-CtERF1 containing 35S promoter. Results: CtERF1 gene had a functional domain of a typical AP2/ERF gene encoding 297 amino acids, and contained an AP2 region speculated to be located in cytoplasm and nucleus, which was ERF subprotein. Systematic evolution analysis showed that CtERF1 gene had some homology with other plant species, among which the relationship with Populus deltoides and Panax japonicus were the closest. The pBASTA-CtERF1 plant expression vector was constructed successfully by molecular biology. Conclusion: A CtERF1 gene of C. tinctorius AP2/ERF transcription factor family was cloned and the plant expression vector pBASTA-CtERF1 was constructed successfully.
