Identification of Fallopia multiflora by allele-specific polymerase chain reaction
10.7501/j.issn.0253-2670.2019.04.024
- VernacularTitle: 何首乌等位基因特异性PCR鉴别方法研究
- Author:
Hui-Qun XIE
1
Author Information
1. School of Pharmacy, Anhui University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Allele-specific PCR;
Fallopia multiflora Thunb.;
Fallopia multiflora Thunb.var. angulata S. Y. Liu;
Molecular identification;
PsbA-trnH
- From:
Chinese Traditional and Herbal Drugs
2019;50(4):951-956
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To establish a rapid molecular identification method for Fallopia multiflora and its adulterants. Methods: Based on psbA-trnH sequences of F. multiflora and its adulterants, the SNP site was searched and the specific primers were designed. The allele-specific PCR amplification of F. multiflora and its adulterants from different producing areas was carried out and the reaction system was optimized. Results: When the annealing temperature was raised to 48 ℃ with 30 cycle number, only the template DNA of F. multiflora could be amplified to obtain the specific 191 bp band whereas the diagnostic PCRs of the other adulterants were all negative. Conclusion: It’s simple and reliable to identify the authenticity of F. multifloa by allele loci specific PCR.
