Identification of miRNA target genes in Panax ginseng by degradome sequencing analysis
10.7501/j.issn.0253-2670.2019.04.023
- Author:
Ying-Fang WANG
1
Author Information
1. School of Traditional Chinese Medicine, Guangdong Pharmaceutical University
- Publication Type:Journal Article
- Keywords:
Degradation sequence analysis;
MiRNA;
Panax ginseng C. A. Mey.;
Shizhu ginseng;
Target gene;
Yuan ginseng
- From:
Chinese Traditional and Herbal Drugs
2019;50(4):945-950
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze and identify ginseng miRNA. Methods: The miRNA and target genes of Shizhu ginseng and Yuan ginseng were detected by the degradation sequencing technology; Functional annotation of Degradome genes was carried out using public databases of KEGG/NR/GO database; The expression of miRNA and target genes of Shizhu ginseng and Yuan ginseng was determinated by real time fluorescence quantitative PCR technique. Results: A total of 13 target genes of eight miRNA families were obtained; The target gene type of miRNA was mainly transcriptional factor, response factor, and signal transduction pathway by means of KEGG/NR/GO database analysis. The results of real time fluorescence quantitative PCR verification of aqc-miR-159, bdi-miR162, cpa-miR319, pgi-miR4376, smo-miR396 and its target genes were basically consistent with the expression of miRNA and target genes from the degradation group. Conclusion: The target genes of partial Panax ginseng miRNA is clarified, which lays the foundation for further study of the possible function of ginseng miRNA.
