Cloning and expressive characteristics of GPS gene and its correlation with saponins content in Eleutherococcus senticosus
10.7501/j.issn.0253-2670.2019.05.029
- Author:
Jia-Zhen ZHANG
1
Author Information
1. Beijing Jingshan School Caofeidian Branch
- Publication Type:Journal Article
- Keywords:
Clone;
Eleutherococcus senticosus (Rupr. et Maxim.) Maxim;
Geranyl pyrophosphate synthetase;
QRT-PCR;
Saponin
- From:
Chinese Traditional and Herbal Drugs
2019;50(5):1227-1231
- CountryChina
- Language:Chinese
-
Abstract:
Objective To clone cDNA sequence of geranyl pyrophosphate synthase (GPS) gene from Eleutherococcus senticosus and analyze genetic characteristics, gene expression level in different organs and the correlation between GPS gene expression and saponins content. Methods RNA was extracted from E. senticosus and reverse transcribed into cDNA. Gene specific primers were designed according to the unigene (c37362.graph_c0) of GPS from transcriptome sequencing data. The full length of the GPS gene cDNA was amplified by PCR. The expression level of GPS gene in different organs was analyzed by qRT-PCR. The content of E. senticosus saponins was detected by spectrophotometry method. Results GPS gene cDNA was cloned from E. senticosus and encodes 419 amino acids with full length of 1 260 bp. GPS protein located in mitochondria does not have transmembrane region. The GPS gene was expressed in each organ and had the highest expression in blade, which is 5.26 times in root. The relative expression of GPS gene and content of saponin showed the same trend and significant positive correlation (r = 0.851, P < 0.05). Conclusion The whole length of cDNA sequence of GPS gene is cloned for the first time, and there is a positive correlation between the expression level of GPS gene and the saponin content of E. senticosus.
