Rapid identification on chemical compounds in roots of rheum nobile based on uplc-esi-hrmsn
10.7501/j.issn.0253-2670.2019.05.007
- Author:
Pan YONG
1
Author Information
1. School of Pharmacy, Southwest Minzu University
- Publication Type:Journal Article
- Keywords:
Anthraquinones;
Phenolic compounds;
Rheum nobile Hook. f. et Thoms;
Tannin;
UPLC-ESI-HRMSn
- From:
Chinese Traditional and Herbal Drugs
2019;50(5):1066-1074
- CountryChina
- Language:Chinese
-
Abstract:
Objective A rapid and efficient UPLC-ESI-HRMSn method was developed and applied to rapidly identify the components in Rheum nobile. Methods With 100% methanol as extract preparation for solution,the extract was separated on an ACQUITY UPLC BEH C18 column (100 mm × 2.1 mm, 1.7 μm), and eluted with a gradient of methanol-water that containing 0.1% formic acid. Chemical constituents were separated and investigated by UPLC-ESI-HRMSn in both positive and negative ion modes. Combined with accurate mass characteristic fragments, retention time and previous literature database, the structures of compounds were identified or tentatively characterized. Results A total of 34 compounds including four anthraquinones, 15 phenolic acid derivatives, 11 tannin precursor and tannins and four organic acid were identified. Twenty-seven compounds were reported for the first time from R. nobile. Conclusion The analytical method of UPLC-ESI-HRMSn was valuable for rapid identification of unknown constituents of R. nobile, which supplies a clear material basis for further study.
