Effects of different drying methods on multiple chemical components in Leonurus japonicus based on UPLC-QTRAP®/MS2
10.7501/j.issn.0253-2670.2019.07.013
- Author:
Ya-Jie TAN
1
Author Information
1. Key Laboratory of Shaanxi Administration of Traditional Chinese Medicine for TCM Compatibility, Shaanxi University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Acteoside;
Ajugol;
Alkaloids;
Apigenin;
Benzoic acid;
Caffeic acid;
Chlorogenic acid;
Drying methods;
Ferulic acid;
Flavonoids;
Genkwanin;
Hesperetin;
Hot-air drying;
Hyperoside;
Infrared drying;
Iridoid glycoside;
Isoquercitrin;
Isorhamnetin;
Kaempferol;
Kaempferol-3-O-rutinoside;
Leonurine;
Leonurus japonicus Houtt.;
Microwave drying;
P-hydroxybenzoic acid;
Phenolic acids;
Phenylpropanoids;
Principal component analysis;
Quercetin;
Rutin;
Salidroside;
Stachydrine;
Syringic acid;
TOPSIS analysis;
Trigonelline;
UPLC-QTRAP®/MS2;
Vanillic acid;
Wogonin
- From:
Chinese Traditional and Herbal Drugs
2019;50(7):1576-1586
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of 15 kinds of drying methods including sun-drying, shade drying, infrared drying (50, 60, 70, 80 ℃), microwave drying (50, 60, 70, 80, 100 ℃), and hot-air drying (50, 60, 70, 80 ℃) on the quality of Leonurus japonicus through the content of multiple chemical components, and then optimize suitable drying methods for L. japonicus. Methods UPLC-QTRAP®/MS2 method was developed to determine the content of three alkaloids (stachydrine hydrochloride, leonurine hydrochloride, trigonelline), four phenolic acids (benzoic acid, p-hydroxybenzoic acid, vanillic acid, syringic acid), five phenylpropanoids (salidroside, acteoside, chlorogenic acid, caffeic acid, ferulic acid), 11 flavonoids (rutin, isoquercitrin, hyperoside, wogonin, kaempferol-3-O-rutinoside, genkwanin, apigenin, kaempferol, isorhamnetin, hesperetin, quercetin), and one iridoid glycoside (ajugol) in L. japonicus. The principal component analysis (PCA) and TOPSIS analysis were performed to evaluate the quality of the L. japonicus samples obtained by different drying methods. Results Different drying methods exerted significant effects on the content of 24 chemical ingredients in L. japonicus. The PCA analysis divided 15 drying methods into three types based on the content of 24 compounds. Moreover, the comprehensive evaluation of TOPSIS was carried out, and the top three drying methods were 70 ℃ hot-air drying, 60 ℃ hot-air drying, and 100 ℃ microwave drying, which largely retained the active ingredients of L. japonicus. Conclusion Combined with practice, we found that 70 ℃ hot-air drying was the optimized drying process of L. japonicus, which provides guarantee for the quality of L. japonicus and provides scientific basis for the production and processing of L. japonicus.
