Lignan constituents from Balanophora involucrate and their anti-inflammatory activities
10.7501/j.issn.0253-2670.2019.08.021
- Author:
Jiang-Chun WEI
1
Author Information
1. School of Traditional Chinese Medicine, Shenyang Pharmaceutical University
- Publication Type:Journal Article
- Keywords:
(-)-secoisolariciresinol-4-O-β-D-gluco- pyranoside;
Anti-inflammatory activity;
Balanophora involucrate Hook. f.;
Dihydrocubebin;
IL-6;
Lignan;
NO
- From:
Chinese Traditional and Herbal Drugs
2019;50(8):1915-1920
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the chemical constituents in the whole herb of Balanophora involucrate. Methods: The compounds were isolated and purified using polyamide, silica gel colimu, ODS, MCI gel, and semi-preparative HPLC, and their structures were elucidated by means of physicochemical properties and spectroscopic analysis. The anti-inflammatory activities of all the isolated compounds were evaluated using Griess method and ELISA for the determination of LPS-induced NO and IL-6 releases in inflammation cell model induced by LPS. Results: Eleven lignans were isolated from 75% ethyl alcohol extract from the whole herb of B. involucrata and identified as (+)-pinoresinol (1), (+)-5’-hydroxypinoresinol (2), isolariciresinol 4-O-β-D-glucopyranoside (3), (+)-isolariciresinol (4), burselignan (5), (+)-9-acetoxyisolariciresinol (6), yunnanensin A (7), (-)-secoisolariciresinol-4-O-β-D- glucopyranoside (8), (-)-secoisolariciresinol (9), dihydrocubebin (10), and secoisolariciresinol-9’-acetate (11). Conclusion: Among them, compound 11 is a new natural product, and compound 2, 5, 7, 8, and 10 are isolated from the genus of Balanophora for the first time. All compounds showed strong anti-inflammatory activities.
