Sini Decoction combined with spectinomycin B1 induced dissociation of PTEN and SUMO1 to treat lung cancer
10.7501/j.issn.0253-2670.2019.09.017
- Author:
Yu-Lan YAN
1
Author Information
1. Department of Hematology Oncology, The Fifth Central Hospital of Tianjin
- Publication Type:Journal Article
- Keywords:
Gene of phosphate and tension;
homology deleted on chromsome ten;
Lung cancer;
Sini Decoction;
Small ubiquitin-related modified protein;
Spectinomycin B1
- From:
Chinese Traditional and Herbal Drugs
2019;50(9):2127-2132
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the mechanism of the inhibition of proliferation, metastasis and the promotion of apoptosis of lung cancer cells by Sini Decoction combined with spectinomycin B1 from the perspective of protein SUMOylation modification. Methods The experiment was divided into control group, spectinomycin B1 group, Sini Decoction group, and combined treatment group. Human non-small cell lung cancer cell line A549 was treated for 48 h and Western blotting was used to detect the protein expression of small ubiquitin-related modified protein-1 (SUMO1), gene of phosphate and tension homology deleted on chromsome ten (PTEN), and phosphorylated Akt (p-Akt) and Caspase-9. Cell proliferation curves were drawn by MTT assay, and cell apoptosis was detected by flow cytometry; Cell migration and cell invasion were detected by cell scratch and Transwell assay. Results Both spectinomycin B1 and Sini Decoction an reduce the levels of covalent SUMO1 and p-Akt, increase the protein levels of PTEN and activate Caspase-9, inhibit cell proliferation, migration and invasion, and promote cell apoptosis. Combined treatment group can further enhance the above changes. Conclusion Sini Decoction can synergize with spectinomycin B1 to induce the dissociation of PTEN and SUMO1, thereby inhibiting the proliferation, metastasis and promoting apoptosis of lung cancer cells.
