Effect of Qi-Boosting Toxin-Resolving Granules and cisplatin on transplanted tumor of nasopharyngeal carcinoma and balance of Foxp3/ROR-γt
10.7501/j.issn.0253-2670.2019.09.016
- Author:
Mei HU
1
Author Information
1. Hunan University of Traditional Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Foxp3;
Nasopharyngeal carcinoma;
Qi-Boosting Toxin-Resolving Granules;
ROR-γt;
Treg/Th17
- From:
Chinese Traditional and Herbal Drugs
2019;50(9):2121-2126
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the immune tolerance of Qi-Boosting Toxin-Resolving Granules (QBTRF) to Treg in the micro- environment of nasopharyngeal carcinoma (NPC) transplanted tumor, and reveal the possible anti-tumor mechanism of traditional Chinese medicine. Methods Using tumor cells CNE2 to build grafted tumor models, the anti-tumor effect of the QBTRF and cisplatin was observed, and the protein expression levels of Foxp3, ROR-γt and the content of IFN-γ, TGF-β and IL-17 in serum were detected. Results It was found that the content of TGF-β and IL-17 in serum of the tumor granule group and the combination therapy group were significantly lower than the model group, the IFN-γ content was obviously higher than that of the model group and cisplatin group, the apoptotic rate was significantly higher than that of the model group, the protein expression of Foxp3 was significantly increased, and the expression of ROR-γt was obviously decreased, in which the combination group was obviously better than that of single-use groups (P < 0.01). Conclusion QBTRF can effectively regulate the balance of Treg/Th17 in mice, restore the function of Treg, enhance the immune function of T cells during chemotherapy in mice, and exert anticancer effect. Combined with cisplatin, it can reduce the toxicity of chemotherapy.
