Process optimization for Rehmanniae Radix Praeparata with yellow wine stewing by multi-index-response surface method
10.7501/j.issn.0253-2670.2019.09.009
- Author:
Bo WANG
1
Author Information
1. Shanxi University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
5-hydroxymethyl-2-furaldehyde;
Comprehensive evaluation;
HPLC;
Leonuridine;
Multiple indicators-response surface method;
Preparation of Chinese materia medica;
Quality standard;
Rehmanniae Radix Praeparata;
Retinoid D;
Stewing with wine;
Verbascoside
- From:
Chinese Traditional and Herbal Drugs
2019;50(9):2065-2073
- CountryChina
- Language:Chinese
-
Abstract:
Objective Based on response surface methodology, the best processing technique for Rehmanniae Radix Praeparata was established in the aspects of multiple evaluations and comprehensive evaluations by the quantitative determination of HPLC. Methods The content of rehmannioside D, ajugol, 5-hydroxymethyl-2-furaldehyde (5-HMF) and verbascoside was used as the evaluation index in the quantitative analysis of HPLC. The response surface methodology was used to investigate the ingestion time of Rehmanniae Radix Praeparata, the adding amount of yellow wine, the stewing time, the drying temperature and the drying time with aim to find the best processing technology. Results The column was a Tnature-C18 column (250 mm × 4.6 mm, 5 μm) with volume flow of 1 mL/min and column temperature of 25 ℃. The elution conditions for the quantitative determination of rehmannioside D and ajugol were as follow: The mobile phase was acetonitrile-0.1% phosphoric acid water (4∶96), and the detection wavelength was 203 nm; That for 5-HMF and verbascoside was as follow: The mobile phase was acetonitrile-0.1% phosphoric acid water (0—15 min, acetonitrile content was 11%; 15—15.1 min, acetonitrile content was 11%—16%; 15.1—50 min, acetonitrile content was 16%) and the detection wavelength was 334 nm. The best technology for Rehmanniae Radix Praeparata with yellow wine stewing was: adding 100 kg of yellow wine 60 kg for infiltrating 12 h, sealing and stewing for 38 h, drying at 76 ℃ for 33 h. Conclusion The established HPLC method was stable and feasible, suitable for the determination of the selected components. The model established by the response surface method was relatively accurate and can be used to predict the content of the four components well. The optimized process with yellow wine stewing method was feasible, which provided a scientific basis for the formulation of quality standards and modern research of Rehmanniae Radix Praeparata.
