Comparative analysis of monoterpene glycosides in Paeoniae Rubra Radix Formula Granule from different manufacturers
10.7501/j.issn.0253-2670.2019.10.012
- Author:
Rui-Xue DENG
1
Author Information
1. College of Chemical Engineering & Pharmaceutical, Henan University of Science and Technology
- Publication Type:Journal Article
- Keywords:
10-hydroxypaeoniflorin;
4-O-methyl-oxypaeoniflorin;
4-O-methyl-paeoniflorin;
Albiflorin;
Albiflorin R1;
Benzoyloxypaeoniflorin;
Benzoylpaeoniflorin;
Formula granule;
Galloylpaeoniflorin;
HPLC;
Monoterpene glycosides;
Mudanpioside F;
Oxyalbiflorin;
Oxypaeonidanin;
Oxypaeoniflorin;
Paeoniae Rubra Radix;
Paeonidanin;
Paeoniflorin;
Pyrindylpaeoniflorin
- From:
Chinese Traditional and Herbal Drugs
2019;50(10):2332-2338
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish an HPLC method for simultaneous determination of 15 monoterpene glycosides pyrindyl- paeoniflorin, mudanpioside F, oxyalbiflorin, oxypaeoniflorin, 10-hydroxypaeoniflorin, albiflorin, paeoniflorin, oxypaeonidanin, 4-O- methyl-oxypaeoniflorin, galloylpaeoniflorin, 4-O-methyl-paeoniflorin, albiflorin R1, paeonidanin, benzoyloxypaeoniflorin, and benzoylpaeoniflorin in Paeoniae Rubra Radix Formula Granule, in order to compare the quality of Paeoniae Rubia Radix Formula Granule from different manufacturers and provide the basis for the establishment of a unified quality control method. Methods The separation was performed on an Agilent Zorbax SB-Aq C18 column (250 mm × 4.6 mm, 5 µm), using acetonitrile and potassium dihydrogen phosphate solution (pH 2.8) as the mobile phase at the flow rate of 1.0 mL/min for a gradient elution. The detection wavelength was set at 260 nm. Results: Paeoniflorin, albiflorin, and oxypaeoniflorin were the three main monoterpene glycosides with the highest content in the Paeoniae Rubra Radix Formula Granule. There were extremely significant difference among the contents of 15 monoterpene glycosides in Paeoniae Rubra Radix Formula Granule produced by different manufacturers. The sample of CSPFKL-KRT was remarkable for the highest content of paeoniflorin and oxypaeoniflorin (73.214 mg and 16.935 mg per gram sample, respectively) and the lowest content of albiflorin among all samples (2.343 mg per gram sample), while sample CSPFKL-XLS was remarkable for the lowest content of paeoniflorin and oxypaeoniflorin (26.327 mg and 4.165 mg per gram sample, respectively) and the highest content of albiflorin among all samples (18.893 mg per gram sample). Conclusion: There were extremely significant difference among the contents of main monoterpene glycosides in Paeoniae Rubra Radix Formula Granule produced by different manufacturers, which may affect the clinical use. The establishment of a unified quality standard plays an important role in the quality control of Paeoniae Rubia Radix Formula Granule.
