Non-polysaccharide chemical constituents of Poria cocos and their anti-complementary activity
10.7501/j.issn.0253-2670.2019.11.004
- Author:
Fu-Yong NI
1
Author Information
1. State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Key Laboratory of New Technique Research of TCM Extraction and Purification, Jiangsu Kanion Pharmaceutical Co., Ltd.
- Publication Type:Journal Article
- Keywords:
Anti-complementary activity;
Lupeol;
Non-polysaccharide chemical constituents;
Oleanolic acid;
Poria cocos (Schw.) Wolf;
Triterpenoid acids;
Ursolic acid
- From:
Chinese Traditional and Herbal Drugs
2019;50(11):2529-2533
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the non-polysaccharide chemical constituents of Poria cocos and their anti-complementary activity. Methods: The anti-complementary bioassay-guided isolation was carried out with the hemolysis test as guide. All isolates were evaluated for their in vitro anti-complementary activities on the classical pathway. The structures were identified by various spectroscopic data including ESI-MS, 1H-NMR, and 13C-NMR data. Results: Eleven compounds were isolated from the EtOAc fraction of P. cocos extracts, including stigmasterol (1), lupeol (2), oleanolic acid (3), ursolic acid (4), polyporenic acid C (5), tumulosic acid (6), dehydrotumulosic acid (7), 3-epi-dehydrotumulosic acid (8), pachymic acid (9), dehydropachymic acid (10), and dehydrotrametenolic acid (11). Compounds 1-4 were obtained from this plant for the first time, and compounds 3-11 showed the anti-complementary activity in different degrees. Conclusion: Triterpenoid acids are the main anti-complementary constituents in the chemical constituents of P. cocos non-polysaccharides (CH50 0.10-0.27 g/L).
