Protective effect of Astragulus polysaccharide on DNA damage in human BM-MSCs exposed to formaldehyde
10.7501/j.issn.0253-2670.2019.12.027
- Author:
Ya-Li SHE
1
Author Information
1. Provincial Level Key Laboratory for Molecular Medicine of Major Diseases and the Prevention and Treatment with Traditional Chinese Medicine Research in Gansu Colleges and Universities, Gansu University of Traditional Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Astragalus polysaccharide;
Bone marrow mesenchymal stem cells;
DNA damage;
DNA repair;
Formaldehyde
- From:
Chinese Traditional and Herbal Drugs
2019;50(12):2928-2933
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the protective effect of Astragalus Polysaccharide (APS) on DNA damage in human BM-MSCs exposed to formaldehyde and to initially explore the potential mechanism. Methods: BM-MSCs were cultured in vitro and divided into control group, formaldehyde group, and APS at 40, 100, and 400 μg/mL groups. Proliferation activity was measured by MTT assay, DNA strand breakage was detected by comet assay, DNA-protein crosslinks (DPCs) was detected by KCl-SDS precipitation assay, and the mRNA and protein expression of XPA, XPC, ERCC1, RPA1 and RPA2 were detected by qRT-PCR and Western blotting. Results: Compared with model group, formaldehyde-contaminated BM-MSCs were treated with APS at 40, 100, and 400 μg/mL, the cell proliferation activity was increased significantly (P < 0.01), DNA strand breakage and DPCs level were decreased significantly (P < 0.01), and the mRNA and protein expression of XPA, XPC, ERCC1, RPA1, and RPA2 were up-regulated significantly (P < 0.05, 0.01). Among them, the effect of 100 μg/mL APS group was the most obvious. Conclusion: APS can protect formaldehyde-induced BM-MSCs DNA damage, especially 100 μg/mL APS has the most obvious effect. The mechanism may be associated with the up-regulation of XPA, XPC, ERCC1, RPA1, and RPA2, which promoted the repair of DNA damage.
