Inhibitory effect of isolinderalactone on growth of human breast cancer MCF-7 cells and its mechanism
10.7501/j.issn.0253-2670.2019.12.026
- Author:
Zi-Yao LIU
1
Author Information
1. School of Life Science, Beijing University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Isolinderalactone;
MCF-7 cells;
Mitochondrial membrane potential;
Proliferation inhibition
- From:
Chinese Traditional and Herbal Drugs
2019;50(12):2922-2927
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effects and the mechanism of isolinderalactone on inhibiting the growth of human breast cancer MCF-7 cells. Methods: Human breast cancer MCF-7 cells were cultured in vitro and treated respectively with indicated concentrations of isolinderalactone by cell culture technique. The proliferation rate was detected by MTT method; Flow cytometry and TUNEL assay were used to observe the effects of isolinderalactone on cell cycle, mitochondrial membrane potential and apoptosis in MCF-7 cells; The apoptosis related protein expression levels were determined by Western blotting. Results: Isolinderalactone significantly inhibited the proliferation of MCF-7 cells by inducing cell apoptosis in a time and concentration dependent manner and induced cell cycle arrest at G2/M phases. The mitochondrial membrane potential was depolarized; And isolinderalactone up-regulated the expressions of apoptosis related proteins Bax and cleaved Caspase-3 and down-regulated the expressions of apoptosis related proteins Bcl-2. Conclusion: Isolinderalactone shows obvious anti-cancer activities by inducing cell apoptosis. The mechanism of inducing apoptosis may be associated with the reduction of mitochondrial membrane potential and activation of caspase pathway.
