HPLC fingerprint of Zhixue Tiaojing Granule and determination of main components
10.7501/j.issn.0253-2670.2019.15.019
- Author:
Shang GAO
1
Author Information
1. Hunan University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Agrimoniae Herba;
Angelicae Sinensis Radix;
Astragali Radix;
Calycosin-7-glucoside;
Codonopsis Radix;
Dipsaci Radix;
Fingerprint;
Formononetin;
HPLC;
Paeoniae Radix Alba;
Paeoniflorin;
Rubiae Radix et Rhizoma;
Zhixue Tiaojing Granule
- From:
Chinese Traditional and Herbal Drugs
2019;50(15):3642-3647
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish an HPLC fingerprint of methanol extract of Zhixue Tiaojing Granule (ZTG), and determine the content of paeoniflorin and calycosin-7-glucosid. Methods: HPLC analysis was performed on an Thermo UltiMateTM U3000 LC system and carried out at 30 ℃ on a column of Thermo AcclaimTM 120 C18 (250 mm × 4.6 mm, 5 μm). Gradient elution system was composed of water solution (phase A) and acetonitrile (phase B). Detection was performed at the wavelength of 254 nm, the mobile flow rate was 1 mL/min, the column temperature was 30 ℃, and sample volumn was 10 μL. Results: The HPLC fingerprint for 10 batches of ZTG were obtained and the similarities for 10 batches of samples were all over 0.95. Eleven characteristic peaks were found and peaks were from Astragali Radix (peak 1, 5, 7, 10), Codonopsis Radix (peak 1), Dipsaci Radix (peak 2, 3), Paeoniae Radix Alba (peak 4), Angelicae Sinensis Radix (peak 6), Rubiae Radix et Rhizoma (peak 6, 8, 11), and Agrimoniae Herba (peak 9). Besides, three peaks were assigned include paeoniflorin, calycosin-7-glucosid, and formononetin. The content of paeoniflorin and calycosin-7-glucosid was 1 062 μg/g and 84.08 μg/g. Conclusion: The method can be used for the quality control of ZTG with good precision, accuracy, and reproducibility.
