Fingerprint analysis of water and ethanol extracts of Glycyrrhiza uralensis from different habitats
10.7501/j.issn.0253-2670.2019.15.008
- Author:
Can PENG
1
Author Information
1. School of Pharmacy, Anhui University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Ethanol extract;
Fingerprinting;
Glycyrrhiza uralensis Fisch.;
Hierarchical cluster analysis;
HPLC;
Similarity-assessing method;
Water extract
- From:
Chinese Traditional and Herbal Drugs
2019;50(15):3569-3574
- CountryChina
- Language:Chinese
-
Abstract:
Objective: The HPLC fingerprints of water extracts and alcohol extracts of Glycyrrhiza uralensis from different producing areas were established in order to provide reference for the quality control and the extraction and processing methods. Methods: The HPLC fingerprints of water extracts and alcohol extracts of 10 batches of G. uralensis from different producing areas were established by HPLC method, and the similarity evaluation and hierarchical cluster analysis of the obtained fingerprint data were carried out. Results: The fingerprint of water extracts and ethanol extracts of G. uralensis was established. There were 16 common peaks were identified from fingerprints of water extracts, and six components were identified, meanwhile, the fingerprints of water extracts showed that the similarity of 10 batches of G. uralensis was in the range of 0.030 and 0.999. Sixty common peaks were identified from fingerprints of ethanol extracts, and seven components were identified, meanwhile, the fingerprints of ethanol extracts showed that the similarity of 10 batches of G. uralensis were all greater than 0.85. The results of hierarchical cluster analysis showed that the water extracts and alcohol extracts of G. uralensis in different areas could be well classified, suggesting that natural factors such as the climate, soil conditions and other natural factors had a significant impact on the internal quality of licorice. Conclusion: A stable and reliable HPLC fingerprint evaluation method for water extracts and alcohol extracts of G uralensis has been established, which can provide a powerful theoretical basis and a guidance for the study of quality standard of G. uralensis and the optimization of extraction and processing technology, and provide a scientific basis for the choice of clinical medication.
