NMR-based study of chemical constituents of Massa Medicata Fermentata
10.7501/j.issn.0253-2670.2019.16.005
- Author:
Hui-Ru ZHANG
1
Author Information
1. School of pharmacy, Liaoning University of Traditional Chinese Medicine
- Publication Type:Journal Article
- Keywords:
(9S,12R,13S)- 9,12,13-trihydroxy-10(E)-octadecenoic acid;
5-heptadecyl resorcinol;
5-pentacosyl resorcinol;
Massa Medicata Fermentata;
Trihydroxy-octadecenoic acid
- From:
Chinese Traditional and Herbal Drugs
2019;50(16):3764-3768
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the chemical constituents from Medicated Leaven (namely Massa Medicata Fermentata in Chinese). Methods: Compounds were isolated and purified from chloroform extract of Medicated Leaven by column chromatograph of silica gel, ODS, Sephadex LH-20, HPLC, and their structure were elucidated on the basis of spectral data and physicochemical property. Results: Eleven chemical compounds were identified as 5-pentacosyl resorcinol (1), 5-heptadecyl resorcinol (2), 5-heneicosyl resorcinol (3), 5-tricosyl resorcinol (4), (9S,10E,12Z)-9-hydroxy-10,12-octadeca-dienoic acid (5), trans-4-hydroxy-2-nonenoic acid (6), 9,10,11-trihydroxy-11(E)-octadecenoic acid (7), (9S,12R,13S)-9,12,13-trihydroxy-10(E)-octadecenoic acid (8), (9S,12S,13S)- 9,12,13-trihydroxy-10(E)-octadecenoic acid (9), (9S,12S,13S)-9,12,13-trihydroxy-10(E)-octadecenoic acid methyl ester (10), and 9,12(Z)-octadecadienoic acid methyl ester (11). Conclusion: All above compounds are all isolated from Massa Medicata Fermentata for the first time, and the chloroform extract of Massa Medicata Fermentata and compound 8 showed inhibitory effect against TNF-α generation of RAW 264.7 induced by LPS in a dose-dependent manner.
