Rapid identification of classical herbal formulae Baoyinjian's chemical substance based by HPLC-Q/TOF-MS
10.7501/j.issn.0253-2670.2019.17.026
- Author:
Wen-Feng MA
1
Author Information
1. Tianjin Medical University
- Publication Type:Journal Article
- Keywords:
Alkaloids;
Baoyinjian;
Flavonoids;
HPLC-Q/TOF-MS;
Iridoid glycosides;
Phenolics
- From:
Chinese Traditional and Herbal Drugs
2019;50(17):4181-4188
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To analyze the chemical constituent cluster of classical herbal formulae Baoyinjian systemically by HPLC-Q/TOF-MS. Methods: The seperation was performed on Diamonsil C18 (250 mm × 4.6 mm, 5 μm) column with gradient elution with acetonitrile-0.1% formic acid. The column temperature was 30 ℃, the flow rate was 1 mL/min, the injection volume was 10 μL, and the mass spectrometry condition was X500R QTOF mass spectrometer, electrospray ion source, positive and negative mode scanning. Results: A total of 52 chemical constituents were identified by reference confirmation, literature comparison, and high mass spectrometry data analysis. The chemical constituent cluster was composed of 17 flavonoids, six phenolics, 12 iridoid glycosides, eight alkaloids, one phenethyl alcohol glycosides, four monoterpene glycoside, two triterpenes and two other compound. Conclusion: This study can identify various chemical constituents of Baoyinjian systematically, accurately, and rapidly, which provides a basis for the determination of the quality attributes of Baoyinjian.
