Simultaneous determination of six anthraquinone components in Arnebia euchroma by QAMS
10.7501/j.issn.0253-2670.2019.17.024
- Author:
Yan MAO
1
Author Information
1. Xinjiang Institute of Material Medica
- Publication Type:Journal Article
- Keywords:
Acetyl-shikonin;
Arnebia euchroma (Royle) Johnstn.;
Deoxyshikonin;
Isobutyrylshikonin;
Multi-component quality control;
Quantitative analysis of multi-components by single marker (QAMS);
Relative correlation factor;
Shikonin;
Β,β'-dimethylacryl shikonin;
Β-acetoxyisovaler- ate shikonin
- From:
Chinese Traditional and Herbal Drugs
2019;50(17):4170-4175
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a new method for the quantitative analysis of multi-components by single-marker (QAMS) to simultaneous determine six naphthoquinone components in Arnebia euchroma. Methods: The chromatographic peaks of the main naphthoquinone components in A. euchroma were identified by high resolution LC-MS. The acetyl Shikonin was used as internal marker to calculate the relative correlation factors (RCF) of deoxyshikonin, isobutyrylshikonin, β-acetoxyisovalerate shikonin, and β,β'-dimethylacryloyl shikonin by HPLC, and examine the durability and reproducibility of the RCF. The external standard method and QAMS were compared to determine the six components in A. euchroma. Results: The repeatability of RCF was good. The results calculated with QAMS were consistent with the results by the external standard method. Conclusion: The QAMS method for simultaneously measuring the content of six components is accurate and reliable to evaluate the quality of A. euchroma.
