Optimization of test solutions preparation of Panax notoginseng in Chinese Pharmacopoeia by Box-Behnken response surface methodology
10.7501/j.issn.0253-2670.2019.17.020
- Author:
Xiao LIANG
1
Author Information
1. School of Pharmacy, Anhui University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Box-Behnken response surface methodology;
Ginsenoside Rb1;
Ginsenoside Rg1;
Notoginsenoside R1;
Panax notoginseng (Burk.) F. H. Chen
- From:
Chinese Traditional and Herbal Drugs
2019;50(17):4145-4151
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study and optimize the preparation method of test solution from Panax notoginseng. Methods: The effects of extraction solvent, liquid-material ratio and extraction temperature on the optimization of the preparation method of test solution of notoginsenoside and ginsenosides in P. notoginseng were investigated by Box-Behnken response surface methology. The content of notoginsenoside R1, ginsenoside Rg1, and ginsenoside Rb1 was simultaneously determined by HPLC. Results: The optimum method was as follow: 75% methanol was used for once reflux extraction, the ratio of liquid-material ratio was 1:40, the extraction temperature was 100 ℃. Conclusion: The optimum preparation of test solution is simple and feasible, and the extraction rate of components is high, which provides a reference for the preparation of test solution from P. notoginseng.
