Dissolution kinetics of Compound Danshen Tablets based on cell bioelectrical sensing effect
10.7501/j.issn.0253-2670.2019.17.018
- Author:
Xiao-Fei MA
1
Author Information
1. School of Pharmacy, Nanjing University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
CCK-8 test;
Compound Danshen Tablets;
Dissolution kinetics model;
In vitro dissolution;
Real-time cell-based assay;
Weibull model
- From:
Chinese Traditional and Herbal Drugs
2019;50(17):4131-4137
- CountryChina
- Language:Chinese
-
Abstract:
Objective: Establishing the model of cell bioelectrical sensing effect of Compound Danshen Tablets to study its dissolution kinetics. Methods: By means of real-time cell-based assay, the in vitro dissolution of Compound Danshen Tablets can be investigated, and then the dissolution kinetics model can also be established. In addition, the result was compared and verified by UV-Vis. Results: The cell line with specific dependence on Compound Danshen Tablets was screened by CCK-8 experiment and RTCA experiment. The dissolution kinetics model of Compound Danshen Tablets based on RTCA technology was established, and the best fitting model was obtained: Weibull model ln{ln[1/(1-Q)] =1.071 4 lnt-3.736 7; Establish a dissolution kinetic model of Compound Danshen Tablets based on UV spectrophotometry to obtain the best fitting model, Weibull model ln{ln[1/(1-Q)]}=1.080 4 lnt-3.723 4; Comparing the two Weibull models, the RTCA fitted model worked better. Conclusion: The application of RTCA in the dissolution kinetics of traditional Chinese medicine compound solid preparations is feasible, Which provides new ideas for traditional Chinese medicines and the quality evaluation of traditional Chinese medicine compunds.
