Expression of GABA Transporters in the Rat's Olfactory Bulb during Development.
- Author:
Yong Jin PARK
1
;
Mun Yong LEE
;
He Ro YOON
Author Information
1. Department of Otolaryngology-Head and Neck Surgery, Korea. yongjinp@yahoo.com
- Publication Type:Original Article
- Keywords:
GABA transporter (GAT-1, GAT-3);
Immunocytochemistry;
Olfactory bulb;
Development;
Rat
- MeSH:
Adult;
Animals;
Axons;
Ependymoglial Cells;
GABA Plasma Membrane Transport Proteins*;
gamma-Aminobutyric Acid*;
Humans;
Immune Sera;
Immunohistochemistry;
Nerve Fibers;
Olfactory Bulb*;
Rats
- From:Journal of Rhinology
1999;6(1):24-29
- CountryRepublic of Korea
- Language:English
-
Abstract:
Developmental changes in the expression of two GABA (gamma-aminobutyric acid) transporter proteins, GAT-1 and GAT-3, in the olfactory bulb of embryonic and postnatal rats were examined with immunocytochemistry using antisera against GAT-1 and GAT-3. The expression and localization of GAT-1 and GAT-3 showed distinct temporal patterns during olfactory bulb development. GAT-1 immunoreactivity appeared weakly in most likely growing axons of the presumptive glomerular layer from embryonic day 18 and increased during the first postnatal week. In contrast, GAT-3 immunoreactivity, first detected at E16, was found in radial glial cell fascicles and was replaced by what were likely astroglial cells postnatally. At P7, GAT-1 and GAT-3 immunoreactivities reached the adult pattern i.e., GAT-1 immunoreactivity was observed in the labeled punctate structures in all layers of the olfactory bulb except the nerve fiber layer, while GAT-3 immunoreactivity was observed in the astroglial processes of all layers of the olfactory bulb. Our results suggest that GABA transporters, especially GAT-3, play important roles in regulating the GABA levels of developing olfactory bulbs.
