Wavelength switching of UPLC fingerprint and chemical pattern recognition of different medical parts of Lonicera japonica
10.7501/j.issn.0253-2670.2019.18.026
- Author:
Ya-Qin ZHEN
1
Author Information
1. Hebei University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Caffeic acid;
Chlorogenic acid;
Cluster analysis;
Cryptochlorogenic acid;
Fingerprint;
Isochlorogenic acid A;
Isochlorogenic acid B;
Isochlorogenic acid C;
Loganin;
Lonicerae Japonicae Caulis;
Lonicerae Japonicae Flos;
Lonicerae Japonicae leaves;
Luteoloside;
New chlorogenic acid;
Principal component analysis;
Rutinum
- From:
Chinese Traditional and Herbal Drugs
2019;50(18):4449-4454
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study Lonicerae Japonicae Flos, Lonicerae Japonicae Caulis, and Lonicerae Japonicae Leaves by UPLC method, and study the different parts of Lonicera japonica by the fingerprint similarity evaluation, cluster analysis, principal component analysis, and other chemical pattern recognition technologies, in order to provide scientific basis for the comprehensive utilization of L. japonica. Methods: The method was carried out on an ACQUITY UPLC BEH C18 column (100 mm × 2.1 mm, 1.7 μm) by a gradient elution using acetonitrile and 0.1% phosphoric acid. The flow rate was 0.3 mL/min, The column temperature was 30 ℃. The sample room temperature was 8 ℃. The detection wavelengths were 326, 238, and 250 nm, and the injection volume was 1 μL. Results: The UPLC fingerprint of 28 batches of samples from different parts of Lonicerae Japonicae were set up and 14 common peaks were obtained. They were new chlorogenic acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, loganin, rutinum, luteoloside, isochlorogenic acid B, isochlorogenic acid A and isochlorogenic acid C. There were some differences in chemical composition and quantity of Lonicerae Japonicae Flos, Lonicerae Japonicae leaves, and Lonicerae Japonicae Caulis. PCA and cluster analysis revealed the similarity and difference of 28 batches of samples from different parts of L. japonica. Conclusion: The combination of clustering analysis and principle component analysis could be used to confirm that the chemical constituents of Lonicerae Japonicae Flos and Lonicerae Japonicae leaves were similar, but there was a difference between Lonicerae Japonicae Flos and Lonicerae Japonicae Caulis. The established fingerprint method can provide a reference for the quality control of Lonicerae Japonicae Flos, Lonicerae Japonicae leaves, and Lonicerae Japonicae Caulis.
