Study on preparation, characterization, and transformation of icariin of immobilized snailase on nano-SiO2
10.7501/j.issn.0253-2670.2019.20.010
- VernacularTitle: 交联纳米SiO2固定化蜗牛酶的制备表征及转化淫羊藿苷研究
- Author:
Rui-Yun LI
1
Author Information
1. Anhui University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Baohuoside I;
Biotransformation;
Cross-linked nano-SiO2;
Icariin;
Immobilized snailase
- From:
Chinese Traditional and Herbal Drugs
2019;50(20):4904-4910
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To prepare the immobilized snailase on cross-linked nano-SiO2, characterize its physicochemical properties, and investigate the optimal conditions of hydrolyzing icariin to baohuoside Ⅰ and enzymatic properties. Methods: The snailase was immobilized on the glutaraldehyde cross-linked nano-SiO2 by covalent coupling method. The immobilization conditions were optimized using the relative enzyme activity as the test index. Transmission electron microscopy (TEM), fourier transform infrared spectroscopy (FTIR), and elemental analysis methods were used to characterize the physicochemical properties of immobilized snailase. Using icariin as the substrate and free enzyme as the control, the optimal enzyme conditions, enzymatic kinetic parameters, recyclability, and thermal stability of the immobilized snailase were also investigated. Results: The optimal mass ratio of enzyme to carrier was 1:3 and the optimal immobilization time was 6 h for the preparation of the immobilized snailase. The optimum hydrolysis conditions of the immobilized snailase were as follows: pH 5.0, conversion temperature 60 ℃, mass ratio of enzyme to substrate 1:2, and transformation time 12 h. The Vmax and Km of the immobilized snailase was 0.43 μg/min and 0.78 mmol/L, respectively. After repeated use for 5 times, the residual relative enzyme activity of the immobilized snailase maintained above 70%. Conclusion: The immobilized snailase on cross-linked nano-SiO2 has high mechanical strength, strong stability, and good reusability. It can hydrolyze the icariin to the baohuoside Ⅰ with a better activity. The hydrolysis process was simple, easy, and suitable for industrial production.
